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Gut microbiota / Evidence-based microbiome knowledge (incl. AI chat)
Prebiotics, Probiotics, FMT / Manipulators of the Intestinal Microbiota

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Shotgun sequencing

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Shotgun sequencing is a general method for sequencing long DNA strands. In this technique, the DNA is broken into smaller fragments, which are then sequenced individually. The sequences of these fragments are then reassembled using computer programs to reconstruct the original sequence.

References:
Hugenholtz et al. 1998 J Bacteriol. 180(18): 4765-4774
Tyson et al. 2004 Nature 428(6978): 37-43
Wooley et al. 2010 PLoS Comput Biol. 6(2): e1000667

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References (Sources)

A primer on metagenomics
Wooley et al. 2010 PLoS Comput Biol. 6(2): e1000667
Review
Community structure and metabolism through reconstruction of microbial genomes from the environment
Tyson et al. 2004 Nature 428(6978): 37-43
Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity
Hugenholtz et al. 1998 J Bacteriol. 180(18): 4765-4774
Review

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5/29/2026

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Shotgun sequencing

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