meRfi®-GM
Analytic Microbiome / Methodology
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Currently, there are numerous barriers that are hindering progress. Researchers in low- and middle-income countries (LMICs) frequently face challenges such as a lack of transport services, freezers, and reliable power supplies, which are essential for obtaining samples and rapidly freezing them at extremely low temperatures (−80 °C or lower). This freezing process is crucial for maintaining the integrity of DNA and RNA for sequencing, as well as ensuring bacterial viability for culturing. Additionally, LMICs often do not have the computational resources necessary to analyze and store large data sets. Access to sealed anaerobic cabinets, which are vital for culturing oxygen-intolerant gut bacteria, archiving the cultured organisms, sequencing their genomes, and characterizing their growth requirements and meta…
References (Sources)
- A human gut microbial gene catalogue established by metagenomic sequencing
- Defining the Human Microbiome
- MHSNMF: multi-view hessian regularization based symmetric nonnegative matrix factorization for microbiome data analysis
- Mikrobiome – Forschung zwischen Theorie und praktischer Anwendung
- Shotgun metagenomics of 250 adult twins reveals genetic and environmental impacts on the gut microbiome
- The function of our microbiota: who is out there and what do they do?
- The gut microbiome’s role in the development, maintenance, and outcomes of sepsisReview