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Vitamin B2 (Riboflavin) is biosynthesized in bacteria (such as Bacillus subtilis)(?id=fe2fca70-9506-5385-9d74-48d118a98349&linkText=bacteria), fungi (e.g., Ashbya gossypii) and plants, not in animals, in multiple steps starting from guanosine triphosphate (GTP) or ribulose-5-phosphate. The essential enzymes are Lumazine synthase, which synthesizes lumazine, and Riboflavin synthase, which condenses two molecules of lumazine derivative to yield…